granulysin-pe (Thermo Fisher)
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Granulysin Pe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/granulysin-pe/pmc11784352-151-49-51
Average 90 stars, based on 1 article reviews
Granulysin Pe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/granulysin-pe/pmc11784352-151-49-51
Average 90 stars, based on 1 article reviews
granulysin-pe - by Bioz Stars,
2026-10
90/100 stars
Images
1) Product Images from "Polycytotoxic T cells mediate antimicrobial activity against intracellular Mycobacterium tuberculosis"
Article Title: Polycytotoxic T cells mediate antimicrobial activity against intracellular Mycobacterium tuberculosis
Journal: Infection and Immunity
doi: 10.1128/iai.00297-24
Figure Legend Snippet: Coculture of sorted T-cell populations and Mtb -infected macrophages: release of effector molecules
Techniques Used: Infection
Figure Legend Snippet: NKG2C + and NKG2A + T-lymphocytes inhibit the growth of intracellular Mtb and contain a higher frequency of P-CTLs. ( A ) Macrophages were infected with Mtb , coated with an anti-CD3 antibody, and cocultured with sorted T-cell subsets for 24 h. Lysates were plated in serial dilutions and the number of CFU was determined after 14 days of incubation. The number of CFU was determined and related to the number of CFU in the culture without T cells (defined as “1.0”). The graph shows the individual values of 13 independent donors (horizontal line: median). Statistical analysis was performed using the Wilcoxon test, two-tailed. ( B ) PBMCs from 30 donors were stained for CD3, CD8, NKG2A, NKG2C, granzyme B, perforin, granulysin, and Live/Dead and analyzed by flow cytometry. The Boolean gating function in FlowJo was used to determine combinatorial expression of granzyme B, perforin, and granulysin within all subsets. The dot plot illustrates the identification of P-CTLs using a subsequent gating strategy. The full gating strategy is depicted in S3. ( C ) The figure shows the frequency of P-CTL in all 30 donors investigated. Statistical comparison was performed with the Wilcoxon test, two-tailed. ( D ) Heatmap presenting the correlation between CTL-subsets and n -fold Mtb growth using Spearman’s rank correlation test ( n = 12). R -values are depicted in the squares. CFU, colony-forming units; Mtb , Mycobacterium tuberculosis , P-CTL, polycytotoxic T-lymphocytes; PBMC, peripheral blood mononuclear cell.
Techniques Used: Infection, Incubation, Two Tailed Test, Staining, Flow Cytometry, Expressing, Comparison
Figure Legend Snippet: Classification of CD3 + CD8 + CTL-subsets
Techniques Used:
Related Articles
Flow Cytometry:Article Title: Induction of granulysin in CD8+ T cells by IL-21 and IL-15 is suppressed by human immunodeficiency virus-1. Article Snippet: Immunosuppression following infection with HIV-1 predisposes patients to a myriad of opportunistic pathogens, one of the most important of which is Mtb.. Granulysin, expressed by NK cells and CTL, exhibits potent antimicrobial activity against Mtb and several other opportunistic pathogens associated with HIV-1 infection.. The immune signals that promote granulysin expression in human CTL are not fully understood. Article Title: Polycytotoxic T cells mediate antimicrobial activity against intracellular Mycobacterium tuberculosis Article Snippet: The following antibodies were used for flow cytometry: CD3-PerCP (clone SK7, BD Biosciences), CD8-BV605 (clone RPA-T8, BioLegend), NKG2A-FITC (clone REA 110, Miltenyi), NKG2A-PE (clone REA 110, Miltenyi), NKG2C-APC (clone REA 205, Miltenyi), granzyme B-Pacific Blue (clone GB11, BioLegend), Recombinase Polymerase Amplification:Article Title: Induction of granulysin in CD8+ T cells by IL-21 and IL-15 is suppressed by human immunodeficiency virus-1. Article Snippet: Immunosuppression following infection with HIV-1 predisposes patients to a myriad of opportunistic pathogens, one of the most important of which is Mtb.. Granulysin, expressed by NK cells and CTL, exhibits potent antimicrobial activity against Mtb and several other opportunistic pathogens associated with HIV-1 infection.. The immune signals that promote granulysin expression in human CTL are not fully understood. Article Title: Polycytotoxic T cells mediate antimicrobial activity against intracellular Mycobacterium tuberculosis Article Snippet: The following antibodies were used for flow cytometry: CD3-PerCP (clone SK7, BD Biosciences), CD8-BV605 (clone RPA-T8, BioLegend), NKG2A-FITC (clone REA 110, Miltenyi), NKG2A-PE (clone REA 110, Miltenyi), NKG2C-APC (clone REA 205, Miltenyi), granzyme B-Pacific Blue (clone GB11, BioLegend), Infection:Article Title: Induction of granulysin in CD8+ T cells by IL-21 and IL-15 is suppressed by human immunodeficiency virus-1. Article Snippet: Immunosuppression following infection with HIV-1 predisposes patients to a myriad of opportunistic pathogens, one of the most important of which is Mtb.. Granulysin, expressed by NK cells and CTL, exhibits potent antimicrobial activity against Mtb and several other opportunistic pathogens associated with HIV-1 infection.. The immune signals that promote granulysin expression in human CTL are not fully understood. Article Title: Polycytotoxic T cells mediate antimicrobial activity against intracellular Mycobacterium tuberculosis Article Snippet: The following antibodies were used for flow cytometry: CD3-PerCP (clone SK7, BD Biosciences), CD8-BV605 (clone RPA-T8, BioLegend), NKG2A-FITC (clone REA 110, Miltenyi), NKG2A-PE (clone REA 110, Miltenyi), NKG2C-APC (clone REA 205, Miltenyi), granzyme B-Pacific Blue (clone GB11, BioLegend), Incubation:Article Title: Induction of granulysin in CD8+ T cells by IL-21 and IL-15 is suppressed by human immunodeficiency virus-1. Article Snippet: Immunosuppression following infection with HIV-1 predisposes patients to a myriad of opportunistic pathogens, one of the most important of which is Mtb.. Granulysin, expressed by NK cells and CTL, exhibits potent antimicrobial activity against Mtb and several other opportunistic pathogens associated with HIV-1 infection.. The immune signals that promote granulysin expression in human CTL are not fully understood. Article Title: Polycytotoxic T cells mediate antimicrobial activity against intracellular Mycobacterium tuberculosis Article Snippet: The following antibodies were used for flow cytometry: CD3-PerCP (clone SK7, BD Biosciences), CD8-BV605 (clone RPA-T8, BioLegend), NKG2A-FITC (clone REA 110, Miltenyi), NKG2A-PE (clone REA 110, Miltenyi), NKG2C-APC (clone REA 205, Miltenyi), granzyme B-Pacific Blue (clone GB11, BioLegend), Two Tailed Test:Article Title: Induction of granulysin in CD8+ T cells by IL-21 and IL-15 is suppressed by human immunodeficiency virus-1. Article Snippet: Immunosuppression following infection with HIV-1 predisposes patients to a myriad of opportunistic pathogens, one of the most important of which is Mtb.. Granulysin, expressed by NK cells and CTL, exhibits potent antimicrobial activity against Mtb and several other opportunistic pathogens associated with HIV-1 infection.. The immune signals that promote granulysin expression in human CTL are not fully understood. Article Title: Polycytotoxic T cells mediate antimicrobial activity against intracellular Mycobacterium tuberculosis Article Snippet: The following antibodies were used for flow cytometry: CD3-PerCP (clone SK7, BD Biosciences), CD8-BV605 (clone RPA-T8, BioLegend), NKG2A-FITC (clone REA 110, Miltenyi), NKG2A-PE (clone REA 110, Miltenyi), NKG2C-APC (clone REA 205, Miltenyi), granzyme B-Pacific Blue (clone GB11, BioLegend), Staining:Article Title: Induction of granulysin in CD8+ T cells by IL-21 and IL-15 is suppressed by human immunodeficiency virus-1. Article Snippet: Immunosuppression following infection with HIV-1 predisposes patients to a myriad of opportunistic pathogens, one of the most important of which is Mtb.. Granulysin, expressed by NK cells and CTL, exhibits potent antimicrobial activity against Mtb and several other opportunistic pathogens associated with HIV-1 infection.. The immune signals that promote granulysin expression in human CTL are not fully understood. Article Title: Polycytotoxic T cells mediate antimicrobial activity against intracellular Mycobacterium tuberculosis Article Snippet: The following antibodies were used for flow cytometry: CD3-PerCP (clone SK7, BD Biosciences), CD8-BV605 (clone RPA-T8, BioLegend), NKG2A-FITC (clone REA 110, Miltenyi), NKG2A-PE (clone REA 110, Miltenyi), NKG2C-APC (clone REA 205, Miltenyi), granzyme B-Pacific Blue (clone GB11, BioLegend), Expressing:Article Title: Induction of granulysin in CD8+ T cells by IL-21 and IL-15 is suppressed by human immunodeficiency virus-1. Article Snippet: Immunosuppression following infection with HIV-1 predisposes patients to a myriad of opportunistic pathogens, one of the most important of which is Mtb.. Granulysin, expressed by NK cells and CTL, exhibits potent antimicrobial activity against Mtb and several other opportunistic pathogens associated with HIV-1 infection.. The immune signals that promote granulysin expression in human CTL are not fully understood. Article Title: Polycytotoxic T cells mediate antimicrobial activity against intracellular Mycobacterium tuberculosis Article Snippet: The following antibodies were used for flow cytometry: CD3-PerCP (clone SK7, BD Biosciences), CD8-BV605 (clone RPA-T8, BioLegend), NKG2A-FITC (clone REA 110, Miltenyi), NKG2A-PE (clone REA 110, Miltenyi), NKG2C-APC (clone REA 205, Miltenyi), granzyme B-Pacific Blue (clone GB11, BioLegend), Comparison:Article Title: Induction of granulysin in CD8+ T cells by IL-21 and IL-15 is suppressed by human immunodeficiency virus-1. Article Snippet: Immunosuppression following infection with HIV-1 predisposes patients to a myriad of opportunistic pathogens, one of the most important of which is Mtb.. Granulysin, expressed by NK cells and CTL, exhibits potent antimicrobial activity against Mtb and several other opportunistic pathogens associated with HIV-1 infection.. The immune signals that promote granulysin expression in human CTL are not fully understood. Article Title: Polycytotoxic T cells mediate antimicrobial activity against intracellular Mycobacterium tuberculosis Article Snippet: The following antibodies were used for flow cytometry: CD3-PerCP (clone SK7, BD Biosciences), CD8-BV605 (clone RPA-T8, BioLegend), NKG2A-FITC (clone REA 110, Miltenyi), NKG2A-PE (clone REA 110, Miltenyi), NKG2C-APC (clone REA 205, Miltenyi), granzyme B-Pacific Blue (clone GB11, BioLegend), |